Lemming diet dataset. Includes samples from 76 lemming winter nests, which plant DNA content was analysed using trnL g/h and c/h primer pairs (Taberlet et al. 2007 Nucleic Acids Research, Vol. 35, No. 3 e14).The dataset consists of:

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Raw sequence files:
Raw sequence fasta file (rodents_2011.fna) and related quality file (rodents_2011.qual). These were processed using software OBITools as described by Soininen et al. 2013 Polar Biology 36:1069-1076.


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Three data files of processed sequence data: 
gh_embl.txt: sequences for primer pair g-h annotated based on embl reference library
gh_arcbor.txt: sequences for primer pair g-h annotated based on arctic and boreal reference library
ch_arcborbryo.txt: sequences for primer pair c-h annotated based on arctic and boreal reference library including bryophytes

all these have following columns:
(i) "sequence_id" identifier of the sequence
(ii) "best_identity" best match with the closest sequence in the reference library 
(iii) "best_match" id of the closest sequence in the reference library
(iv) "sample:246" to "sample:325" occurence of sequence in samples identified by sample number
(v) "scientific_name" scientific names of sequence in reference library
(vi) "sequence" DNA sequence


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A datafile for sample characteristics: "Lemming_sample_data.txt" with following columns:
(i) "sample ID" sample number
(ii) "tag_ch" tag DNA sequence used for this sample together with the c-h primer pair
(iii) "tag_gh" tag DNA sequence used for this sample together with the g-h primer pair
(iv) "habitat" habitat type where sample was collected; mesic, wetland or riparian
(v) "species obs" sample lemming species identity based on visual observation
(vi) "species dna" sample lemming species identity based on dna analysis 
(vii)"amplicon_size"	detected size of discrimination amplicon
(viii) "sample_type" distinguishing between feces analysed for diet or muscle samples analysed for verifying detection of lemming species identity based on mitochondrial Cytochrome Oxydase I COI 
Data that is not available / not collected for specific type of samples is denoted with NA.

Contact author: 
Eeva Soininen (eeva.soininen@uit.no, eeva.soininen@gmail.com)