File megafauna_sequence_data.fasta This file contains the unique sequences produced by amplification of DNA from permafrost samples using the 16Smam1 and 16Smam2 primers. Amplicons were sequenced using Roche 454 GS FLX Titanium. Tags, primers and reads less than 10 bp in length have been removed and all exactly identical reads are merged. Samples were collected at Main River, Siberia (64.28 lat , 171.25 lon), and date from 47 to 20 kyr BP. Further details on sample collection, DNA extraction, PCR conditions, and tools used for filtering of the raw sequence data can be found in the associated publication. Contact author: Sanne Boessenkool (sanneboessenkool@gmail.com) The header of each sequence contains: (i) the ID for the sequence (ii) the total number of occurrence of the sequence in the dataset (count) (iii) the number of occurrence in the different samples (merged-sample) Below is a list of extraction numbers (EH and SL - corresponding to the numbers in the fasta file), followed by the sample collection number: Sample EH0075_S1_mam: 57A Sample EH0279_S1_mam: 31 Sample EH0280_S1_mam: 35 Sample EH0282_S1_mam: 57 Sample EH0293_S1_mam: 11 Sample EH0294_S1_mam: 12 Sample EH0295_S1_mam: 52 Sample EH0296_S1_mam: 53 Sample EH0300_S1_mam: 10B Sample EH0310_S1_mam: 45 Sample EH0311_S1_mam: 44 Sample EH0315_S1_mam: 40 Sample EH0317_S1_mam: 34A Sample EH0320_S1_mam: 38 Sample EH0338_S1_mam: 25 Sample EH0339_S1_mam: 20 Sample EH0341_S1_mam: 14 Sample EH0343_S1_mam: 60 Sample EH0344_S1_mam: 61 Sample EH0345_S1_mam: 13 Sample EH0994_S1_mam: 32A Sample EH0995_S1_mam: 50A Sample EH1026_S1_mam: 49A Sample EH1027_S1_mam: 51 Sample SL042_S1_mam: 39A