FastQCFastQC Report
Mon 8 May 2023
NC-EB_S18_L002_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNC-EB_S18_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49874
Total Bases4.9 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTAATCTCGTAT998820.02646669607411TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTAATCGCGTAT37327.482856799133818TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCATG2240.44913181216666TruSeq Adapter, Index 12 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTAATCTCGTATGCC2240.44913181216666TruSeq Adapter, Index 12 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTAATCTCGTTT1710.3428640173236556TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTAATCTCGGAT1160.2325861170148775TruSeq Adapter, Index 12 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTAATCGCGGAT980.19649516782291374TruSeq Adapter, Index 12 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTAATCGCGTATGCC870.17443958776115812TruSeq Adapter, Index 12 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGGCTAATCGCGTTT780.15639411316517626TruSeq Adapter, Index 12 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)