FastQCFastQC Report
Mon 29 Apr 2024
Sk_22_001_EB_S24_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSk_22_001_EB_S24_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10526
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCATGCATGCCGCTGTCGTTGACCACTATCATTATACGTACGTAGATC2162.0520615618468554No Hit
ATGCATGCATGCCGCTGTCGTTGACCACTATCATTATACGTACGTAAGAT1651.5675470264107922No Hit
GGTGGTTCCGGAGGATCCCTGAGCGCGAGCCCGCGCATGAGCGGCTTTAT890.8455253657609728No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG880.8360250807524225No Hit
AGGGTGAACCTGGGGGAGTATTGCGGAGGAAGGTGCTGTGCTGAGATCGG850.8075242257267717No Hit
AGGGTGAACCTGGGGGAGTATTGCGGAGGAAGGTGCTGTGCTAGATCGGA470.4465133954018621No Hit
ACGGGATCCGGAGGCGGAGGCTCGGGAGGCGGAGGTACCTGCCCGGAAGG430.40851225536766106No Hit
GGTGGTTCCGGAGGATCCCTGAGCGCGAGCCCGCGCATGGCGGGCTTTAT280.2660079802394072No Hit
ACGTACGTATAATGATAGTGGTCAACGACAGCGGCATGCATGCATAGATC230.2185065551966559No Hit
CGTGGATCCACCTCCGCCTCCCGAGCCTCCGCCTCCAAGATTCTTGCCTT230.2185065551966559No Hit
TACGTACGTATAATGATAGTGGTCAACGACAGCGGCATGCATGCATAGAT170.16150484514535435No Hit
GTGGTTCCGGAGGATCCCTGAGCGCGAGCCCGCGCATGAGCGGCTTTATT130.12350370511115334No Hit
GTGGATCCACCTCCGCCTCCCGAGCCTCCGCCTCCAAGATTCTTGCCTTC110.10450313509405282No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)