FastQCFastQC Report
Mon 29 Apr 2024
Sk_22_001_EB_S24_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSk_22_001_EB_S24_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10526
Total Bases1.5 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCATCTCGTAT3403.230096902907087TruSeq Adapter, Index 19 (97% over 38bp)
ACGTACGTATAATGATAGTGGTCAACGACAGCGGCATGCATGCATAGATC2142.033060991829755No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCATCGCGTAT1731.6435493064791946TruSeq Adapter, Index 19 (97% over 38bp)
TACGTACGTATAATGATAGTGGTCAACGACAGCGGCATGCATGCATAGAT1601.520045601368041No Hit
CAGCACAGCACCTTCCTCCGCAATACTCCCCCAGGTTCACCCTAGATCGG870.8265247957438724No Hit
CGCCCTTGCTCACCAATTGTTTGCCCTGATAAATAAAGCCGCTCATGCGC670.6365190955728672No Hit
AGCACAGCACCTTCCTCCGCAATACTCCCCCAGGTTCACCCTAGATCGGA460.4370131103933118No Hit
GCCCTTGCTCACCAATTGTTTGCCCTGATAAATAAAGCCGCTCATGCGCG350.33250997529925896No Hit
CGTGGATCCACCTCCGCCTCCCGAGCCTCCGCCTCCAAGATTCTTGCCTT340.3230096902907087No Hit
ACGGGATCCGGAGGCGGAGGCTCGGGAGGCGGAGGTACCTGCCCGGAAGG280.2660079802394072No Hit
ATGCATGCATGCCGCTGTCGTTGACCACTATCATTATACGTACGTAGATC230.2185065551966559No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCATCTCGTATGCC170.16150484514535435TruSeq Adapter, Index 19 (97% over 35bp)
ATGCATGCATGCCGCTGTCGTTGACCACTATCATTATACGTACGTAAGAT160.1520045601368041No Hit
GTGGATCCACCTCCGCCTCCCGAGCCTCCGCCTCCAAGATTCTTGCCTTC150.14250427512825384No Hit
CGCCCTTGCTCACCAATTGTTTGCCCTGATAAATAAAGCCCGCCATGCGC140.1330039901197036No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGTTAAGGCATCGCGTATGCC120.11400342010260307TruSeq Adapter, Index 19 (97% over 35bp)
GCCCTTGCTCACCAATTGTTTGCCCTGATAAATAAAGCCCGCCATGCGCG110.10450313509405282No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGA110.10450313509405282Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)