FastQCFastQC Report
Mon 29 Apr 2024
Sk_22_001_C6_S23_L001_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSk_22_001_C6_S23_L001_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2224
Total Bases333.6 kbp
Sequences flagged as poor quality0
Sequence length150
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCATGCATGCCGCTGTCGTTGACCACTATCATTATACGTACGTAGATC1928.633093525179856No Hit
ATGCATGCATGCCGCTGTCGTTGACCACTATCATTATACGTACGTAAGAT1346.025179856115108No Hit
AGGGTGAACCTGGGGGAGTATTGCGGAGGAAGGTGCTGTGCTGAGATCGG1004.496402877697841No Hit
GGTGGTTCCGGAGGATCCCTGAGCGCGAGCCCGCGCATGAGCGGCTTTAT542.4280575539568345No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG401.7985611510791366No Hit
AGGGTGAACCTGGGGGAGTATTGCGGAGGAAGGTGCTGTGCTAGATCGGA351.5737410071942446No Hit
ACGGGATCCGGAGGCGGAGGCTCGGGAGGCGGAGGTACCTGCCCGGAAGG261.169064748201439No Hit
ACGTACGTATAATGATAGTGGTCAACGACAGCGGCATGCATGCATAGATC210.9442446043165468No Hit
CGTGGATCCACCTCCGCCTCCCGAGCCTCCGCCTCCAAGATTCTTGCCTT200.8992805755395683No Hit
GGTGGTTCCGGAGGATCCCTGAGCGCGAGCCCGCGCATGGCGGGCTTTAT140.6294964028776978No Hit
GTATCATCATCATCACTGTCTCCCATAAAAACTACAGATCGGAAGAGCGT110.49460431654676257No Hit
CCCAAAGAGGAAAATTTGTTTCATACAGGAGATCGGAAGAGCGTCGTGTA70.3147482014388489Illumina Single End PCR Primer 1 (100% over 21bp)
GGAGTTCAGACGTGTGCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGG70.3147482014388489Illumina Multiplexing Index Sequencing Primer (100% over 27bp)
TACGTACGTATAATGATAGTGGTCAACGACAGCGGCATGCATGCATAGAT70.3147482014388489No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGATCGGAAGAGCGTCGTGTAGG40.1798561151079137Illumina Single End PCR Primer 1 (96% over 29bp)
ATGCATGCATGCCGCTGTCGTTGCCACTATCATTATACGTACGTAGATCG40.1798561151079137No Hit
GGAGTTCAGACGTGTGCTCTTCCAGATCGGAAGAGCGTCGTGTAGGGAAA40.1798561151079137Illumina Single End PCR Primer 1 (100% over 27bp)
GACCACATGATCCGTGAGCGCATCGCGCGCGACGGCGATGTGCGCCCGAT40.1798561151079137No Hit
CCTCTTGGGAGAGAGGCACTAGTCAAAAACATGAATGTGTGTGCTGATGA30.13489208633093525No Hit
GCCCTTGCTCACCAATTGTTTGCCCTGATAAATAAAGCCGCTCATGCGCG30.13489208633093525No Hit
GGTGGTTCCGGAGGATCCCTGAGGCGAGCCCGCGCATGAGCGGCTTTATT30.13489208633093525No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTAGATCGGAAGAGCGTCGTGT30.13489208633093525Illumina Single End PCR Primer 1 (96% over 30bp)
TGTGCTCGGGGCCCTGGCCTTCGTAGCCGTGCGGCAGCATCAGCGTCAGG30.13489208633093525No Hit
GAATAGAAAGCCAGGAAATAAGGCTACACATCTATAACAATCTGATCTTT30.13489208633093525No Hit
GGAGTTCAGACGTGTGCTCTTCAGATCGGAAGAGCGTCGTGTAGGGAAAG30.13489208633093525Illumina Single End PCR Primer 1 (100% over 28bp)
ATCACCGACTGCCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTT30.13489208633093525Illumina Single End Sequencing Primer (100% over 33bp)
AAACTCTAAATTCTCGTTGACTTTTTTCATATTAAAATCAATAAAAAGAT30.13489208633093525No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)