FastQCFastQC Report
Mon 29 Apr 2024
Sk_22_001_C6_S23_L001_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSk_22_001_C6_S23_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2224
Total Bases333.6 kbp
Sequences flagged as poor quality0
Sequence length150
%GC60

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATTCGATCTCGTAT27912.544964028776977TruSeq Adapter, Index 22 (97% over 37bp)
ACGTACGTATAATGATAGTGGTCAACGACAGCGGCATGCATGCATAGATC1908.543165467625899No Hit
TACGTACGTATAATGATAGTGGTCAACGACAGCGGCATGCATGCATAGAT1335.98021582733813No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATTCGATCGCGTAT1034.631294964028776TruSeq Adapter, Index 22 (97% over 37bp)
CAGCACAGCACCTTCCTCCGCAATACTCCCCCAGGTTCACCCTAGATCGG994.451438848920864No Hit
CGCCCTTGCTCACCAATTGTTTGCCCTGATAAATAAAGCCGCTCATGCGC441.9784172661870503No Hit
AGCACAGCACCTTCCTCCGCAATACTCCCCCAGGTTCACCCTAGATCGGA351.5737410071942446No Hit
ACGGGATCCGGAGGCGGAGGCTCGGGAGGCGGAGGTACCTGCCCGGAAGG281.2589928057553956No Hit
ATGCATGCATGCCGCTGTCGTTGACCACTATCATTATACGTACGTAGATC210.9442446043165468No Hit
CGTGGATCCACCTCCGCCTCCCGAGCCTCCGCCTCCAAGATTCTTGCCTT190.85431654676259No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATTCGATCTCGGAT130.5845323741007195TruSeq Adapter, Index 22 (97% over 37bp)
CGCCCTTGCTCACCAATTGTTTGCCCTGATAAATAAAGCCCGCCATGCGC130.5845323741007195No Hit
GCCCTTGCTCACCAATTGTTTGCCCTGATAAATAAAGCCGCTCATGCGCG110.49460431654676257No Hit
GTAGTTTTTATGGGAGACAGTGATGATGATGATACAGATCGGAAGAGCAC110.49460431654676257No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATTCGATCTCGTTT90.40467625899280574TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGA80.3597122302158274Illumina Multiplexing PCR Primer 2.01 (96% over 29bp)
CCTGTATGAAACAAATTTTCCTCTTTGGGAGATCGGAAGAGCACACGTCT70.3147482014388489Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATGCATGCATGCCGCTGTCGTTGACCACTATCATTATACGTACGTAAGAT60.2697841726618705No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATTCGATCGCGTTT50.22482014388489208TruSeq Adapter, Index 22 (97% over 37bp)
GGTGGTTCCGGAGGATCCCTGAGCGCGAGCCCGCGCATGAGCGGCTTTAT40.1798561151079137No Hit
ATGGGCAGTCGGTGATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC40.1798561151079137Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGAACTC40.1798561151079137Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
ACGTACGTATAATGATAGTGGCAACGACAGCGGCATGCATGCATAGATCG40.1798561151079137No Hit
GTGGATCCACCTCCGCCTCCCGAGCCTCCGCCTCCAAGATTCTTGCCTTC40.1798561151079137No Hit
CTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTCTGA40.1798561151079137Illumina Single End PCR Primer 1 (96% over 27bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATTCGATCTCGTATG40.1798561151079137TruSeq Adapter, Index 22 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATTCGATCGCGGAT40.1798561151079137TruSeq Adapter, Index 22 (97% over 37bp)
AGGTTGAGACACGGGATCTGTTCCACGCTCTCCATCGGGCGCACATCGCC40.1798561151079137No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCCCCAAAGAGGAAAATTTTT30.13489208633093525TruSeq Adapter, Index 7 (97% over 35bp)
GTGAAGTCAAGTGGGGCCGCATCAACGGCCTGACGCTGATGCTGCCGCAC30.13489208633093525No Hit
TTTTATTGATTTTAATATGAAAAAAGTCAACGAGAATTTAGAGTTTAGAT30.13489208633093525No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTATTCGATCGCGTATG30.13489208633093525TruSeq Adapter, Index 22 (97% over 36bp)
CTTTAAGTCAACCTTGAAAGTAAGAATAAACAGTTCATCAGCACACACAT30.13489208633093525No Hit
GAAGAGCACACGTCTGAACTCCAGATCGGAAGAGCACACGTCTGAACTCC30.13489208633093525Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCTTTCCCTACACGACGCTCTTCCGATCAGATCGGAAGAGCACACGTC30.13489208633093525Illumina Single End PCR Primer 1 (96% over 30bp)
GAAGAGCACACGTCTGAACTCCAGTCACCGTATTCGATCTCGTATGCCGT30.13489208633093525TruSeq Adapter, Index 22 (96% over 32bp)
TATATTTTTCCCTATGGATAATTGCCCCAGCACTATTTACTGAAAAGTCA30.13489208633093525No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCGTATTCGATCTCGTATGCC30.13489208633093525TruSeq Adapter, Index 22 (97% over 34bp)
TCCAGGATAGCATTGCCTAGGTTGTATTCTAGAGTTTTTATTGTTTTGGG30.13489208633093525No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)