FastQCFastQC Report
Mon 8 May 2023
NC-EB_S18_L002_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNC-EB_S18_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49874
Total Bases4.9 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC67

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8431.6902594538236355No Hit
GCTCTTCCGATCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCACAC2200.4411116012351125Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)